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In this protocols what is the volume of bacterial growth media...

jayalekshmi haripriyan Aug 13, 2014

Protocol: Extraction and Quantification of Cyclic Di-GMP from Pseudomonas aeruginosa

In this protocols what is the volume of bacterial growth media used for the cultivation of P.aeruginosa

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Karin Sauer Author Answered Sep 12, 2022

Biological Sciences Department, Binghamton University, State University of New York at Binghamton, USA

We generally grow Pseudomonas aeruginosa in 250ml flasks with shaking, using 25-50 ml of growth medium. For biofilms, we used a tube reactor system, and collected biofilms as a paste into microfuge tubes. What is important is that for c-di-GMP extraction, similar ODs are being used. For all samples regardless of growth conditions, we made use of 1 ml adjusted to an OD600 = 1.8

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