Improve Research Reproducibility A Bio-protocol resource

Sorry, I have a question, when you preparing your Lysis buffer...

HAILONG ZHANG
HAILONG ZHANG Aug 11, 2014
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells
Sorry, I have a question, when you preparing your Lysis buffer and Wash buffer, what's the pH value for the 0.1M Na2HPO4/NaH2PO4? because different pH value needs different ratio between these two reagents.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
3 answers
Sort by: most helpful / most recent

Vanesa Olivares-Illana Author Answered Aug 12, 2014

Instituto de Física, Universidad Autónoma de San Luis Potosí

yes, indeed for lysis buffer pH should be 8 and for wash buffer should be 6.8. Hope you have a nice experiment!!
0 helpful
0 unhelpful
comment 0 comments down up
share Share

HAILONG ZHANG Answered Aug 11, 2014

Neoletix Lab, Inc

Thanks Vanesa,Let me make sure for the Lysis buffer: the pH should be the same as Tris-Hcl? so the 0.1M Na2HPO4/NaH2PO4 pH should be 8 in Lysis buffer and 6.8 in Wash buffer? or both are the same pH:7.0? Please confirm.Thank you again.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Vanesa Olivares-Illana Author Answered Aug 11, 2014

Instituto de Física, Universidad Autónoma de San Luis Potosí

Hello, the pH should be 8
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer