In your step "9", pre-equilibrate beads with lysis buffer, the...

HAILONG ZHANG
HAILONG ZHANG
Aug 11, 2014
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells
In your step "9", pre-equilibrate beads with lysis buffer, the last volume should be 75ul but not 75ml.
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HAILONG ZHANG Answered Aug 11, 2014

Neoletix Lab, Inc

Thanks for your reply
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Vanesa Olivares-Illana Author Answered Aug 11, 2014

Instituto de Física, Universidad Autónoma de San Luis Potosí

yes! indeed is 75ul
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