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Improve Research Reproducibility
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10. ...
Jul 31, 2014
Protocol:
Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
10. 过滤器的下侧细胞迅速由5%戊二醛固定10分钟(用结晶紫染色也需要固定吗?为什么固定方法各有不同,还有4%多聚甲醛,甲醛,和戊二醛,请问这些固定方法有区别吗)
11. 过滤器的下侧的细胞用含有1%结晶紫的2%的乙醇染色20分钟。(不是用0.1%的结晶紫染色吗?)
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