How can I set up culture from PBMC?1- I separate blood using...
How can I set up culture from PBMC?
1- I separate blood using ficoll
2- used RPMI+ 10%FBS+ Lglu+ strep/pencellin
3- culture the cell in 96 well plate (10000 cells/well) and in flask at 1000000 cell/ml
the cells is not growing and the counting is down every d Read more Read less

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