How can I set up culture from PBMC?1- I separate blood using...

ma
mohamed ashour
Jul 24, 2014
protocol Protocol: In vitro Culture of Human PBMCs
How can I set up culture from PBMC?

1- I separate blood using ficoll
2- used RPMI+ 10%FBS+ Lglu+ strep/pencellin
3- culture the cell in 96 well plate (10000 cells/well) and in flask at 1000000 cell/ml

the cells is not growing and the counting is down every d
Read more Read less more less
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share

0 answer

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer