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Hi, Thank you for your protocol, I have a question: in step...

HAILONG ZHANG
HAILONG ZHANG Jul 21, 2014
protocol Protocol: Calcium Phosphate Transfection of Eukaryotic Cells
Hi, Thank you for your protocol, I have a question: in step "9" you mix the two solutions at room temperature for 20-30 minutes. but I see some other protocols including your references saying 1 minute is enough. prolonged incubation would decrease the DNA binding efficient.
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Yanling Chen Author Answered Jul 25, 2014

The Scripps Research Institute

The incubation time needs to be determined empirically for different cell types. Yes, a shorter incubation time can be used. Thanks.
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