For the first step, why we have to place in cold 90% Acetone...

protocol Protocol: Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
For the first step, why we have to place in cold 90% Acetone on ice, (why Acetone, why ice?)
Thank you so much!
Please answer as much details as possible, I'm just a 3rd year of Bachelor :)
Thank again
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Xiyan Li Author Answered Jun 23, 2014

Department of Genetics, Stanford University, USA

Cold acetone is for fixation, a way to preserve the cell/tissue structures as in vivo.
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