For running SDS for collagen, how do i prepare my sample? i...

Did they ever get a Collagen protocol made? I am interested in one as I am working with Collagen I currently. Thanks

Hi again, Sorry, I wrote the wrong email, it is katica.carreno@gmail.com.

Thank you!

Katerine Carreño

Hi Fanglian, I'm interested in collagen separation on a SDS-PAGE gel too! Can you please pass my request to Bio-protocol Editorial Committee? I'll appreciate it so much!

Best regards,
Katerine Carreño
katerine.carreno@gmail.com

hi dear.
How can i prepare the sample buffer of 20 ml for the yeast secreted proteins seperation. Please mention the percentage of each components. Can I add B-mercaptoethanol during sample buffer preparation or I need to add during sample preparation?. thanks

Hello! I'm also interested in collagen identification through SDS-page!Could you please recommend me such reference as well, if you please? Thank you so much!

Kaidu Hanashiro Barrosa,
kaiduhb@gmail.com

Hi, Sorry I do not have any experience with running collagen on a SDS-page gel. But, I have passed your request to Bio-protocol Editorial Committee. They will recommend/invite a research group to contribute the requested protocol to Bio-protocol in the near future. Will keep you posted on this.

--Fanglian