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Do you have a try even lower temperature, for example 15°C,...

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liu chunxia Jun 11, 2014
protocol Protocol: The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
Do you have a try even lower temperature, for example 15°C, 16°C?
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Hogune Im Author Answered Jun 18, 2014

Molecular and Cellular Pharmacology Program, Department of Pharmacology, University of Wisconsin Medical Schoo, USA

No, I don't think it would make a huge difference but it will grow significantly slower.
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