Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
when we prepare some vectors, some fragments have toxin genes...
HD
Hongjiu Dai May 27, 2014
when we prepare some vectors, some fragments have toxin genes and sequences. My may be hard to get vector amplification in E.coli. Do you think your super competent cells could solve this problem, thanks
5
Views
Answer
Recommend
Follow
Share
1
answer
Sort by: most helpful / most recent
Xiyan Li Author Answered May 27, 2014
Department of Genetics, Stanford University, USA
No, i won't help.
What you need is a suitable host strain that us more tolerant.
I would also try low copy number plasmid and low culture temperature, e.g. 30C or even 25C.
What you need is a suitable host strain that us more tolerant.
I would also try low copy number plasmid and low culture temperature, e.g. 30C or even 25C.
0 helpful
0 unhelpful
0 comments ShareMy answer
Write your answer...
References (optional)
Add more
Post an Answer