Dear Sir Thanks a lot for repeated cooperation and your valuable...

SI
Shilpi Islam
May 26, 2014
protocol Protocol: Analysis of Mouse Liver Glycogen Content
Dear Sir
Thanks a lot for repeated cooperation and your valuable suggestions. I could not understand that glucosyl unit containing only glycogen or ester of glycogen (glycogen + glucose). I need to express my result as the concentration of glucose mg / g or glycogen mg/g wet tissue f
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Peichuan Zhang Answered Jun 11, 2014

Department of Biology, The Pennsylvania State University, USA

Hi Shilpi,

Acid hydrolysis of glycogen will yield glucose monomers, which will be converted further into G-6-P by hexokinase in the kit. The glucosyl unit, as expressed in the equation, refers to a glucose monomer. 1 umole of glucosyl unit is roughly 180ug (D-glucose, MW=180.2).
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