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Dear SirThanks a lot for your valuable information. I followed...
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Shilpi Islam May 22, 2014
Dear Sir
Thanks a lot for your valuable information. I followed your protocol as per your recommendation. In normal condition, sheep liver glycogen concentration is below of 100 mg/g wet liver wt. But my glycogen value was 1200-1500 mg/g wet liver wt.which too large. I boiled about 20 Read more
Thanks a lot for your valuable information. I followed your protocol as per your recommendation. In normal condition, sheep liver glycogen concentration is below of 100 mg/g wet liver wt. But my glycogen value was 1200-1500 mg/g wet liver wt.which too large. I boiled about 20 Read more
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Peichuan Zhang Answered May 23, 2014
Department of Biology, The Pennsylvania State University, USA
Hi Shilpi, we typically analyzed liver glycogen levels for mice that had been fasted overnight. I'd recommend you to check the feeding status for your study subjects, which would have strong effects on their liver glycogen contents. Meanwhile, could you please check and make sure that you use the right amount of glucose standard for the calculation?
Best,
Peichuan
Best,
Peichuan
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