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Question:We have been staining monocytes from PBMCs and staining...

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Anna Trier May 21, 2014
protocol Protocol: Whole Blood Staining of Human Monocyte Subsets for Flow Cytometry
Question:

We have been staining monocytes from PBMCs and staining has been working. Recently we switched to whole blood staining for patient samples (so we could take less blood). However, now our classical monocytes (CD14++CD16-) appear to have CD16 expression (compared to the Read more Read less more less
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Zheng Liu Author Answered Mar 9, 2015

Bio-protocol

Please try to stain before lysis. The double negative cells could be contamination from other population. We try to gate them out using a dump gate containing T, B, NK cell markers, but you always get a few of those cells regardless.
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