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Dear sirI am Shilpi Islam. Thank you for your previous solution....
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Shilpi Islam May 11, 2014
Dear sir
I am Shilpi Islam. Thank you for your previous solution. I have another inquire that after hemolysis of liver sample supernatant hydrolyzed solution will be used for the glycogen content. Can I store that supernatant solution for further use? If that is possible, so, what co Read more
I am Shilpi Islam. Thank you for your previous solution. I have another inquire that after hemolysis of liver sample supernatant hydrolyzed solution will be used for the glycogen content. Can I store that supernatant solution for further use? If that is possible, so, what co Read more
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Peichuan Zhang Answered May 12, 2014
Department of Biology, The Pennsylvania State University, USA
Hi Shilpi,
This is a good question. Typically, I'd conduct the HK glucose assay right away once when I have obtained the glycogen hydrolysis product. 10 minutes of boiling is sufficient to kill the enzymes that could possibly degrade glucose, the molecule of our interest. However, long-term storage (for example, @ -20C or -80C with repeated cycles of freezing/thawing) may increase oxidation of glucose to gluconolactone, and thus, bring more deviations to the final assay. I'd recommend you to store the products (protect them from light) @ 4C if you need to wait to have all the samples ready for the assays.
Best,
Peichuan
This is a good question. Typically, I'd conduct the HK glucose assay right away once when I have obtained the glycogen hydrolysis product. 10 minutes of boiling is sufficient to kill the enzymes that could possibly degrade glucose, the molecule of our interest. However, long-term storage (for example, @ -20C or -80C with repeated cycles of freezing/thawing) may increase oxidation of glucose to gluconolactone, and thus, bring more deviations to the final assay. I'd recommend you to store the products (protect them from light) @ 4C if you need to wait to have all the samples ready for the assays.
Best,
Peichuan
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