Hi, can u suggest me the proper extraction buffer for leaf samples...

protocol Protocol: Laemmli-SDS-PAGE
Hi, can u suggest me the proper extraction buffer for leaf samples for coomassive briliant blue staining for sds page method. Can u also give me instruction how to prepare the extraction buffer.
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Fanglian He Author Answered Apr 28, 2014

Bio-protocol

I am not sure what kind of plant leaf you are interested in.

For Arabidopsis leaf, you may check out the following protocol.

Flury et al. (2013). MAPK Phosphorylation Assay with Leaf Disks of Arabidopsis. Bio-protocol 3(19):http://www.bio-protocol.org/wenzhang.aspx?id=929

For tobacco leaf,
Husk et al. (2014). Monoclonal Antibody Purification (Nicotiana benthamiana Plants).Bio-protocol 4(2):http://www.bio-protocol.org/wenzhang.aspx?id=1034

Both above protocols have details about how to extract proteins from leaves including the recipes of the extraction buffers.Hope you would find them helpful.
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