Hello, i just want to ask a few questions:1. how did you made...

Protocol: Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast

Hello,

i just want to ask a few questions:

1. how did you made the 10% digitonin solution (in water, ethanol...)?
2. for the IP buffer are all the components required (what is the use of sorbitol, NaF#, Na3VO4, CaCl...)?

I tested my digitonin from S Read more Read less

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