The concentration of IPTG specified for induction is approximately...

protocol Protocol: Purification of 6x His-tagged Protein (from E. coli)
The concentration of IPTG specified for induction is approximately 1000-times higher than required. Final concentration of 1 mM IPTG should be more than sufficient for induction. (I would assume the authors used 0.6 mM)

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Bio-protocol Editorial Team Answered Mar 11, 2014

bio-protocol.org

Hi Alexander,

Thank you for pointing out. With author's permission, we have corrected it in the text accordingly.

--Bio-protocol Editorial Board
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