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Can we carryout this experiment with Fluorescent microscope?...

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sudipa galgalkar Feb 26, 2014
protocol Protocol: Detection of Membrane Potential in Mycobacterium tuberculosis
Can we carryout this experiment with Fluorescent microscope? If so then which excitation/emission wavelength should i select
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sudipa galgalkar Answered Mar 17, 2014

siemens

Many Thanks Manbeena!!
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Amit Singh Author Answered Mar 14, 2014

Immunology Department, International Centre for Genetic Engineering and Biotechnology, India

Yes this experiment can be carried out with fluorescent microscopy. The green fluorescence of DIOC2(3) requires 488 nm excitation and 530 nm emission while the red fluorescence requires 488 nm excitation and 610 nm emission. Hope that will help!
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