HelloI appreciate from your protocol, your assistant and your...

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farzane sadeghi
Feb 20, 2014
protocol Protocol: Alcian Blue – Alizarin Red Staining of Mouse Skeleton
Hello
I appreciate from your protocol, your assistant and your guide.
I did yor protocol and gain what I want.
But a question :
In trypsin stage my samples that were 30 olds mice clear after 4 days but you told that 1 day is enough for clearing stage.
why?
T
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Peichuan Zhang Answered Feb 24, 2014

Department of Biology, The Pennsylvania State University, USA

Hi Farzane,

This variation could be due to difference in the efficiency of trypsin digest. Try to adjust the procedures accordingly, as the protocol here serves to provide basic guide line.

Thanks,
P

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