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Hi, is the membrane is permeable to collagen? If so, the...

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xl w Feb 19, 2014
protocol Protocol: Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
Hi, is the membrane is permeable to collagen? If so, the concentration of collagen will equilibrate between the up and low compartment. How collagen can still work as chemattractants?
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Yanling Chen Author Answered Feb 21, 2014

The Scripps Research Institute

This experimental setup allows gradient of chemoattractant be established, which is the "driving force" for migration. We don't know how long it takes to "equilibrate" if that would happen eventually, time is also a determining factor here, I think.
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