HelloI want to know that temperature is important in trypsin...

Hi Farzane,

We just soaked the samples in the trypsin solution overnight at room temperature (not at 37 degrees) --- this should work well enough.

For your other question --- we used ethanol instead of formaldehyde to fix embryos. Maybe this helps to avoid over-fixing. I'd recommend you to open the abdomen of the embryos with scissors after sacrificing them in your experiments.

Best,
P