Dear Dr Ito-Ishida,I am interested in obtaining details on your...

protocol Protocol: Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation
Dear Dr Ito-Ishida,
I am interested in obtaining details on your ex vivo electroporation protocol for cerebellar progenitors.
I would really appreciate if you could answer some of my doubts. Please feel free to send me any other detail you consider relevant.
- we had a ha
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Aya Ito-Ishida Author Answered Feb 7, 2014

Department of Cellular Neurobiology, Graduate School of Medicine, University of Tokyo, Japan

Dear Dr. Silveira,
1. I have never used Hamilton syringe for this purpose myself, but I think it is possible. You may need to practice several times so that the plasmid-containing liquid will go into the fissures slowly. It may be easier to try it with another person's help: one person could hold the cerebellum and another person could manipulate the syringe.
2. When injecting the plasmid-containing liquid, the tip of the capillary has to be within the cerebellar fissures. The two cerebellar lobules will make walls on both sides of the capillarly tip and prevent the liquid from diffusing. In addition, the electric pulses should be applied right after the injection.
_ I hope this helps. Please let me know if you have any further questions.
Best wishes,
Aya Ito-Ishida
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