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Hi,I notice that you don't use detergents in the lysis buffer....
ME
Marie-Laure Erffelinck Jan 21, 2014
Hi,
I notice that you don't use detergents in the lysis buffer. Is there a reason for this ?
Greetings,
M-L
I notice that you don't use detergents in the lysis buffer. Is there a reason for this ?
Greetings,
M-L
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Xiyan Li Author Answered Jan 21, 2014
Department of Genetics, Stanford University, USA
Common lab detergents, such as Tween and Triton, are PEG polymers. They are very bad for LC-MS analysis. Some glucoside-based detergents might be useful in this case. I had success with n-dodecyl glucoside in preparing membrane proteins such as yeast PMA1.
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