Great paper, this is really a useful technique! 2 quick questions:...

Protocol: miRNA Tagging and Affinity-purification (miRAP)

Great paper, this is really a useful technique! 2 quick questions: 1) For step 3d, do you remove the beads before adding the acid phenolchloroform? Or do you add it directly to the bead/RNA mixture? and 2) Have you ever tried any commercially available RNA extraction kits instead of the ph Read more Read less

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