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I have seen Tumorspheres with invert-microscopy,but i can't...

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tara fani Dec 20, 2013
protocol Protocol: In vitro Tumorsphere Formation Assays
I have seen Tumorspheres with invert-microscopy,but i can't determine their size,I was wondering if you'd mind helping me,what method or device can i use to determine their size(50-200µm)?
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Ting Yu Answered Jun 19, 2016

Sichuan University

Dear Sara,
How to identify the range of diameter for tumorspheres? 50-250 micrometers?
Thanks!
Ting Yu
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Sara Johnson Author Answered Dec 30, 2013

University of South Carolina

Dear Tara,

When counting and photographing the spheres, we use the imaging software that accompanied our Olympus inverted scope (DP2-BSW software). This program has a 100 micrometer scale bar that allows us to estimate the size of the tumorspheres.

I hope that this helps to answer your question,
Sara
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