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The cells (Hep 3B) will die to 40~50% when we sub-cultured...

Shih-Hsien Hsu Dec 18, 2013

Protocol: Soft Agar Anchorage-independent Assay

The cells (Hep 3B) will die to 40~50% when we sub-cultured it at secondary time at the 700ng/ml G418 dosage. The cells will die to 80~90% at the third subculture. The death cells will dependent on the transfection efficiency. If not so, I suggest you had checked you Hep 3B cells and It m Read more more