I read your paper and it is interesting. But, how to establish...

Protocol: Soft Agar Anchorage-independent Assay

I read your paper and it is interesting. But, how to establish stable cell lines expressing desired gene with vectors ? How to select Hep 3B to stable clones? When I treated Hep 3B cells with G418 from 100 to 1100ug/ml and maintained 7 days(change once 3 days),the cells did not die out.Th Read more Read less

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