Thank you for sharing this protocal. I have read the user manual...

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Qingqing Wei
Dec 6, 2013
protocol Protocol: Single-cell Gene Expression Profiling of Mouse Stem Cells With Fluidigm BiomarkTM Dynamic Array
Thank you for sharing this protocal.
I have read the user manual of The CellsDirectTM One-Step qRT-PCR kit and have a question to consult.
The kit contains Lysis Solution (Resuspension buffer and Lysis Enhancer), but The 2x Reaction Mix provided in this kit only consists of a pr
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Ana Sevilla Answered Dec 17, 2013

Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, USA

Hi Quingqing
For the lysis, Fluidigm technical support suggested to use only the 2X Reaction buffer from the CellsDirectTM One-Step qRT-PCR kit and I never got issues related with incomplete lysis.
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