Thank you for sharing this protocal. I have read the user manual of The CellsDirectTM One-Step qRT-PCR kit and have a question to consult. The kit contains Lysis Solution (Resuspension buffer and Lysis Enhancer), but The 2x Reaction Mix provided in this kit only consists of a prRead moreRead less
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, USA
Hi Quingqing For the lysis, Fluidigm technical support suggested to use only the 2X Reaction buffer from the CellsDirectTM One-Step qRT-PCR kit and I never got issues related with incomplete lysis.