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HiI work with yeast and I have got polysomes fractions from...

sa
sara ali Nov 19, 2013
protocol Protocol: Polysome Preparation, RNA Isolation and Analysis
Hi
I work with yeast and I have got polysomes fractions from my colleque and I want to converst them to cDNA using RT_PCR kit
Can you please help me wich primer should I use??
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HAILONG ZHANG Author Answered Nov 24, 2013

Neoletix Lab, Inc

Hi, Sara, we used the kit "iScript? Reverse Transcription Supermix for RT-qPCR" from Bio-rad for reverse transcription, please see the link for more detailed information:
https://www.bio-rad.com/en-us/sku/170-8840-iscript-reverse-transcription-supermix-for-rt-qpcr

Good luck
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