Hi, I have a silly question. When I pull down protein of my...

JM
Jana M
Nov 5, 2013
protocol Protocol: A General Protocol for GST Pull-down
Hi,
I have a silly question. When I pull down protein of my interest with my gst-fusion protein bound to glutathion sepharose 4b beads (for example TLR3-GST beads) what do I use as control beads? Is it glutathion sepharose 4b slurry (naked beads) or glutathion sepharose beads bound t
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Lili Jing Author Answered Nov 8, 2013

Department of Cell and Molecular Biology, University of Pennsylvania, USA

It should be GST beads.
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