Improve Research Reproducibility A Bio-protocol resource

Hello,Thank you for this helpful protocol. In step 6, should...

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Thomas Dohlman Oct 21, 2013
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Hello,

Thank you for this helpful protocol.
In step 6, should the new media contain anti-CD28?

Thank you!
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Jia Li Author Answered Oct 25, 2013

SRI International

No need to add anti-CD28.
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