Hello sirplease explain the calculations because i cannot understand...

Thank you for the question. The same volume of hydrolysis products and 0.5mM glucose standard solution were used in the final enzymatic assay, and the concentration of glucosyl groups in the hydrolysis product is calculated by normalization of the absorbance of that of the 0.5mM globose. Then the total amount of glucosyl units in the 1.0ml hydrolyzed liver sample is determined, and then used for the calculation of average glucosyl units.

For example, 10mg liver was hydrolyzed and dissolved in 1.0ml solution.
Abs 10ul sample = 0.4
Abs 10ul 0.5mM glucose = 0.8

Then the total amount of glucosyl units in this 1.0ml solution will be:
0.4/0.8 x 0.5 x 1 = 0.25 umol
The average would be 0.25/10 = 0.025 umol glucosyl units/ mg wet liver

I hope this have answered your question.

Best,
Peichuan