Lili Jing Author Answered Oct 14, 2013
Department of Cell and Molecular Biology, University of Pennsylvania, USA
Hey,
I haven't tried to do this myself. But I have heard the following from other people and you may give it a try.
After the myotubes are well formed (usually after 5 days), Aspirate medium, wash with PBS, add trypsin, check myotube detach under microscope. When only myotubes but not un-differentiated cells detach, quicly collect the trypsined myotubes. It needs only 1 to 2 min for myotubes detach.