After differentiating the myoblasts into myotubes, how would...

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Petey H
Oct 14, 2013
protocol Protocol: Culture, Differentiation and Transfection of C2C12 Myoblasts
After differentiating the myoblasts into myotubes, how would you remove the non-differentiated myoblasts in order to study only myotubes?
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Petey H Answered Oct 16, 2013

UCSF

Hello, thank you. I think that is a good idea, and I will give it a try.
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Lili Jing Author Answered Oct 14, 2013

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Hey,

I haven't tried to do this myself. But I have heard the following from other people and you may give it a try.
After the myotubes are well formed (usually after 5 days), Aspirate medium, wash with PBS, add trypsin, check myotube detach under microscope. When only myotubes but not un-differentiated cells detach, quicly collect the trypsined myotubes. It needs only 1 to 2 min for myotubes detach.
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