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can i use sodium or potassium acetate in place of ammonium acetate?....

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cecilia ojesola Oct 10, 2013
protocol Protocol: Yeast Genomic DNA Miniprep Using A FastPrep Cell Lyser
can i use sodium or potassium acetate in place of ammonium acetate?.
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Xiyan Li Author Answered Oct 10, 2013

Department of Genetics, Stanford University, USA

Potassium is almost replaceable with ammonium as cations. The difference here is the pH. NH4Ac is naturally at pH7.0 without any adjustment. But you have to add acid to KAc for pH7.0, which changes the overall concentration of acetate.
Besides, ammonium acetate is volatile, thus "cleaner" than potassium for downstream applications.

Xiyan
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