Hi,I've read the reference-papers and in both of them TrisHCL...

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simone bosso
Oct 6, 2013
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells
Hi,I've read the reference-papers and in both of them TrisHCL concentration in Lysis and Wash Buffers is 10mM not 100mM as written above: which is the actual TrisHCL concentration?

I've another question: why is the volume of lysis buffer requested for NiNTA beads incubation (5m
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Bio-protocol Editorial Team Answered Oct 24, 2013

bio-protocol.org

Per author's request, the concentration of Tris-HCl has been corrected to 10mM in the protocol.

Sincerely,
Bio-protocol editing team
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Vanesa Olivares-Illana Author Answered Oct 7, 2013

Instituto de Física, Universidad Autónoma de San Luis Potosí

yes you are completely right! the good concentration is 10mM, im sorry for this mistake.
On the other hand 5ml is the volume that for my proteins gave the best yield
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