Hi,I've read the reference-papers and in both of them TrisHCL concentration in Lysis and Wash Buffers is 10mM not 100mM as written above: which is the actual TrisHCL concentration?
I've another question: why is the volume of lysis buffer requested for NiNTA beads incubation (5mRead moreRead less
Instituto de Física, Universidad Autónoma de San Luis Potosí
yes you are completely right! the good concentration is 10mM, im sorry for this mistake. On the other hand 5ml is the volume that for my proteins gave the best yield