Improve Research Reproducibility A Bio-protocol resource

Thank you very much for sharing the protocol. If the L929 cells...

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chunxiao hu Oct 4, 2013
protocol Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Thank you very much for sharing the protocol. If the L929 cells cultivated in the same medium for 5 days, the cell condition is not very good. Will this influnce the living of the BMM. And how to get L-cell conditioned medium of high quality?
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Ran Chen Author Answered Oct 5, 2013

Zhejiang JFK Biological Technology Co. Ltd.

That will definitely affect the living of the BMM. If the L929 cells are not in good conditions and you still want to use the conditioned medium of this not-good conditions, you can try to increase the amount of the conditioned medium in the BMM medium to 20%-30%.
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