Have you tried Xtreme Gene HP to transfect these cells? Roche has replaced Fugene products with XtremeGene products. I was wondering if Xtreme Gene HP works the same as Fugene HD? Roche recommends 70-90% confluency before transfecting with XtremeGene HP, but maybe 50% would be better? If yRead moreRead less
Department of Cell and Molecular Biology, University of Pennsylvania, USA
Hi, I haven't tried Xtreme Gene HP. I would think it will work. For growing C2C12 cells, it is better to be low confluency. The cells udergo differentiation very easily once they are confluent.