Have you tried Xtreme Gene HP to transfect these cells? Roche...

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Becky Diebold
Oct 1, 2013
protocol Protocol: Culture, Differentiation and Transfection of C2C12 Myoblasts
Have you tried Xtreme Gene HP to transfect these cells? Roche has replaced Fugene products with XtremeGene products. I was wondering if Xtreme Gene HP works the same as Fugene HD? Roche recommends 70-90% confluency before transfecting with XtremeGene HP, but maybe 50% would be better? If y Read more Read less more less
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Lili Jing Author Answered Oct 5, 2013

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Hi, I haven't tried Xtreme Gene HP. I would think it will work.
For growing C2C12 cells, it is better to be low confluency. The cells udergo differentiation very easily once they are confluent.
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