Dear Sara, Hexin and Pang-Kuo, I just have a technical question...

protocol Protocol: In vitro Tumorsphere Formation Assays
Dear Sara, Hexin and Pang-Kuo,

I just have a technical question concerning the tumorsphere formation assay.
Are you using 96-well Ultra-low Attachment Plates with flat or round bottom ? Because, I tried out the one's with a round bottom, and I think that all my cells, are
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Hexin Chen Author Answered Oct 1, 2013

Department of Biological Science, University of South Carolina, USA

Dear Melanine,

We are using the flat-bottom plate. The cells are likely to be attacked to the edge but they should be able to form sphere well.

Best,

Hexin
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