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I have this question: in order to obtain the input (starting...

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Giuseppe Bosso Sep 21, 2013
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells

I have this question: in order to obtain the input (starting material/whole cell extract) can I save 15-30ul of lysate before diluting it with 4ml more of lysis buffer and beads incubation? Many Thanks
Giuseppe.
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Vanesa Olivares-Illana Author Answered Sep 26, 2013

Instituto de Física, Universidad Autónoma de San Luis Potosí

Hello and sorry for the delay in the answer!!. Yes you can keep 15-30ul of the lysate.
cheers
Vanesa
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