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Interestingly, there is a parallel discussion of this protocol...

Fanglian He
Fanglian He Sep 7, 2013
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Interestingly, there is a parallel discussion of this protocol on ResearchGate: http://www.researchgate.net/post/Naive_T_cell_from_mouse_does_not_go_through_in_vitro_th1_th2_any_suggestion

--Fanglian
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