how do i interprete the gel after destaining, i mean the...

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cecilia ojesola
Aug 20, 2013
protocol Protocol: Laemmli-SDS-PAGE
how do i interprete the gel after destaining, i mean the bands
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Fanglian He Author Answered Aug 20, 2013

Bio-protocol

I am not quite sure what your question is. In general, while looking at your gel, the first question is:
where is the band for your protein of interest (POI)?
--Based on the sizes of makers and known MW of POI, you should know where the band of POI is expected to be.
--If the expression level of POI is very low, you may try some sensitive staining (like silver staining)
--I'd also mention that one single band on SDS-PAGE could consist of more than one protein, which have similar MW. You can do 2D-gel to further separate it.
--It would be very helpful if you have controls to confirm your results. Best way to confirm you result is to do Western Blot using ABs against POI.

Hope I have answered your question.
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