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I used this protocol, however my cells did not go through...

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Louie kwang Aug 7, 2013
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
I used this protocol, however my cells did not go through differentiation. after 7 days, I barely see less 1% INFg in Th1 and IL4 in Th2. any suggestion.
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Jia Li Author Answered Aug 7, 2013

SRI International

I think maybe your intracellular staining does not work
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