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Hi,I'm having some troubles reproducing some knockdown results....
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larita salv Aug 4, 2013
Hi,
I'm having some troubles reproducing some knockdown results.
Sometimes, independently of scratch width, my scratched cells migrate very poorly and after more than 48h wound is still open and cells alive.
Does it ever happen to you? Any advice?
Another question: do Read more
I'm having some troubles reproducing some knockdown results.
Sometimes, independently of scratch width, my scratched cells migrate very poorly and after more than 48h wound is still open and cells alive.
Does it ever happen to you? Any advice?
Another question: do Read more
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Yanling Chen Author Answered Aug 6, 2013
The Scripps Research Institute
Outcome of the "healing" process very much depends on the cell type, i.e., those that migrate aggresively/spread out quickly probably will heal the gap much faster, such as the example cell line mentioned in the protocol. Also, the "healing" process is resulted from not only cell migrating, but also cell growth. finally, please select enough views and repeat the same assay several times-just to reduce variability in readouts so that they are objective and reliable (statistically).
And I don't think sodium pyruvate would have negative effect on would healing.
Hope that these could help.
And I don't think sodium pyruvate would have negative effect on would healing.
Hope that these could help.
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