Hi Hani,Thanks for the protocol.I was wondering if you have...

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Wil
Aug 2, 2013
protocol Protocol: Measurement of Liver Triglyceride Content
Hi Hani,

Thanks for the protocol.

I was wondering if you have tried this protocol to measure plasma triglyceride levels as well? If not, would you expect it to also work?

Thanks
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Hani Jouihan Author Answered Aug 2, 2013

Division of Endocrinology, Gerontology and Metabolism, Stanford University School of Medicine, USA

Hi Wil
I would assume it should work, especially that you have already separated the plasma, so no blood-cells would interfere with the reading. However, I never tested plasma.
If you decided to try the protocol with plasma, make sure to include a positive control (from liver, for example) and used several different volumes of plasma so that your values are withing the linear range of the standard curve.
Good luck
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