请教一下,封闭液为什么要用MAB buffer?1%BSA + 8%serum + 1%triton...

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Jul 30, 2013
protocol Protocol: pH3 Antibody Staining Protocol for Zebrafish
请教一下,封闭液为什么要用MAB buffer?1%BSA + 8%serum + 1%triton X100可以吗?
PFA之后还用丙酮固定一遍,这是为什么呢?如果不固定会怎么样?
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