Hi,Thanks for the protocol. In my experiment i am not seeing...

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Pritha Ray
Jul 19, 2013
protocol Protocol: A Protocol for Electrophoretic Mobility Shift Assay (EMSA) from Primary Neuron
Hi,
Thanks for the protocol. In my experiment i am not seeing any trace of the free labeled probe at the bottom of the gel though a retarded band is present at high molecular weight. This band can be competed out with excess cold probe. I tried running a very short gel by keeping brom
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Jiali Li Author Answered Jul 19, 2013

Rutgers University

Please make sure whether a correct DNA-running gel is used. Is any positive control set up?
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