Hi, I would like to know why the collagen I were added in the...

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Lisha Chen
Jul 2, 2013
protocol Protocol: Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
Hi, I would like to know why the collagen I were added in the medium in the lower compartment. I saw some experimental procedures say the uupper chambers should be pre-coated with rat collagen type I.What's the function of the rat tail collagen I in this assay? Thank you!
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Yanling Chen Author Answered Aug 13, 2013

The Scripps Research Institute

I guess that should work too as long as you can get it dissolved well.
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Lisha Chen Answered Aug 13, 2013

SINANO

Thank you!I have another question. Is the 2% ethanol in crystal violet solution necessary? If I use the solution that crystal violet dissovled in ddH2O, can it work?
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Yanling Chen Author Answered Jul 19, 2013

The Scripps Research Institute

Collagen is added as a chemoattractant; you may use other chemoattractants of your choice.
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