急求回答上面的问题,谢谢

Julia 丽娜
Julia 丽娜
Jun 5, 2013
protocol Protocol: Immunofluorescence (Especially for Cells Growing on a Coverglass)
急求回答上面的问题,谢谢
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Hui Zhu Author Answered Jun 14, 2013

Stanford University

After spin down the secondary antibody stock, take the supernatant, and make the working solution. The procedure won’t destroy the antibody, it can get rid of precipitates in the stock which may cause fluorescence background.
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