Hi, I would like to know whether I should coat some ECM like...

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Lisha Chen
May 28, 2013
protocol Protocol: Scratch Wound Healing Assay
Hi, I would like to know whether I should coat some ECM like Matrigel or other proteins? Some articles I have read recommed protein coating before experiment. Thank you!
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Yanling Chen Author Answered Jun 4, 2013

The Scripps Research Institute

I think switching to serum-free medium should not be a problem as long as your cells can survive and grow well (for some time) in the serum-free medium. But please keep in mind that some cell lines need serum to maintain normal metabolism and other functions such as spreading out or moving etc.
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Lisha Chen Answered May 31, 2013

SINANO

Thank you! I have another question. Should I change the medium to the serum free medium before I scratch the line? Since I need to detect a cytokine activity. When I detect the proliferation activation, I always change to a serum free medium to starve the cell before add the cytokine. Thank you!
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Yanling Chen Author Answered May 29, 2013

The Scripps Research Institute

You may coat the surface with some proteins as long as the protein layer is even and uniform. For Matrigel, I think it's more difficult to scratch the surface to generate a gap, and the cells may grow into the EMC and stay there (not grow to "health" the "gap"). Please try and find a better and creative way for your experiments.
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