how to interpret the results of this assay?

protocol Protocol: Cell Adhesion Assay

how to interpret the results of this assay?






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Yanling Chen Author Answered Jul 16, 2013

The Scripps Research Institute

There are actually tons of references for cell adhesion assay. For background information and methods, please see the references(and the references therein) below as examples. I would always recommend to start with some simple tests then make necessary modifications to these protocols.

Liotta et al. Nature2001; 411: 375–9
Fashena et al. Nat Cell Biol2000; 2: E225–9.
Chen etal. Cancer Res2009 69; 3713
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prashant kurkute Answered Jun 5, 2013

NIPER

Hi yalling ,

I want to do cell adhesion study with my synthesize peptide.
I read your protocol, I am decide to use it , would you please suggest me the paper
from where I get more information on the protocol and how to interpret result ???

Thank you in advance.
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Yanling Chen Author Answered Apr 15, 2013

The Scripps Research Institute

Cell adhesion is a complex process and different factors involved in this process would contribute to the final results. For interpretation of the results, it really depends on what you are looking for and how your experiment is designed. Take just a simple (and ideal) example, if a chemical is capable of directly interfering with cell receptor-ECM interactions, less cell adhering to the surface would be expected to be seen. Most chemicals/proteins may alter cell adhesion by certain indirectly ways, possibly through changes in different cell signaling pathways and remodeling of the cytoskeletal components. Therefore cause must be taken to design and carry out experiments, as well as interpretation of the readouts.
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